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Implantation of the biodegradable polymer matrix "SpheroGel" and embryonic stem cells under experimental complete spinal injury.
The 7th International Congress of the Cell Transplant Society. November 17 to 20-2004. Boston. USA
A Brukhovetski. V.Yaryghin, V. Chekhonin. E. Savchenko, V. Sevastyanov. N. Perova, S. Abrosimov.
Interventional and Restorative Neurology and Therapy Clinic "NeuroVita";
Russian State Medical University;
State Research Centre of Social and Legal Psychiatry after V.R Serbski;
Scientific Research Institute of Transplantology and Artificial Organs;
Moscow. Russia.
Introduction:
Recovery of the spinal functions after injuries is one of the biggest
challenges to modern medicine. It is hardly possible to meet this challenge
without modeling reconstructive spinal surgeries. In this experiment
we applied the technology of spinal reconstruction through the biodegradable
polymer "SpheroCel" with embryonic ensheathing stem cells.
Methods:
Embryonic ensheathing glioolfactory stem cells, received from olfactory
bulbs of rat embryos were applied in the experiment. Stem cells were
cultivated by G. Raisman et al. method (1998], Eighty rats of Vistar
line were pre-clinically tested through complete spinal injury modeling.
The I" group of 20 rats (control group) undergone laminectomy on D5 level,
opening of dura mater, excision of 3 mm of spinal cord and consecutive
layered closure. The biodegradable polymer matrix "SpheroCel" was implanted
in the diastasis zone of the 2" group (30 rats) and the biodegradable polymer
matrix 'Spherogel" with ensheathing neuroepithelial stem cells of rats was
implanted in the 3" group (30 rats). No immunosuppression was applied.
Results:
In two months 5% of the control 1st) group recovered spinal
functions; in the 2nd group 56% recovered lost functions and in the 3d
group 86% regained partial or complete motor activity and control
of pelvic functions. The results were verified by neurophysiologic,
morphologic and immunochemical tests of spinal cord.
Conclusion:
We proved that in case of special conditions created in the zone
of complete spinal injury, injured spinal axons can regenerate,
cyst and scar formation can be considerably reduced
and neurological deficit can be largely corrected.
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